韩国大邱庆北科学技术院（DGIST）Dae Won Moon研究组的一项最新研究发现使用单层石墨烯可对溶液中未处理的湿细胞膜进行质谱成像。该研究于2021年2月4日发表于国际学术期刊《自然-方法学》杂志。
研究人员开发了一种方法，可以通过单层石墨烯溅射原子和分子的次级离子，包括胆固醇和脂肪酸，使次级离子质谱（SIMS）在溶液亚细胞空间分辨率下对未处理的湿细胞膜成像。研究人员可以观察未经处理湿细胞膜中脂质的固有分子分布，例如胆固醇、磷脂酸乙醇胺和各种脂肪酸，而无需任何标记。
研究表明，在具有细胞培养基湿基质上制备的石墨烯覆盖细胞是存活的，并且它们的细胞膜在超高真空环境SIMS成像过程中不会崩解。从头计算分子动力学和离子剂量依赖性研究表明，单层石墨烯溅射是通过在石墨烯层中产生瞬时孔完成的。胆固醇成像表明甲基-β-环糊精优先从细胞膜中富含胆固醇的区域摄取胆固醇分子。
附：英文原文
Title: Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene
Author: Heejin Lim, Sun Young Lee, Yereum Park, Hyeonggyu Jin, Daeha Seo, Yun Hee Jang, Dae Won Moon
Issue&Volume: 2021-02-04
Abstract: We report a means by which atomic and molecular secondary ions, including cholesterol and fatty acids, can be sputtered through single-layer graphene to enable secondary ion mass spectrometry (SIMS) imaging of untreated wet cell membranes in solution at subcellular spatial resolution. We can observe the intrinsic molecular distribution of lipids, such as cholesterol, phosphoethanolamine and various fatty acids, in untreated wet cell membranes without any labeling. We show that graphene-covered cells prepared on a wet substrate with a cell culture medium reservoir are alive and that their cellular membranes do not disintegrate during SIMS imaging in an ultra-high-vacuum environment. Ab initio molecular dynamics calculations and ion dose-dependence studies suggest that sputtering through single-layer graphene occurs through a transient hole generated in the graphene layer. Cholesterol imaging shows that methyl-β-cyclodextrin preferentially extracts cholesterol molecules from the cholesterol-enriched regions in cell membranes. Application of a single layer of graphene to untreated wet mammalian cells enables mass spectrometry imaging of cellular membranes of live cells in solution at subcellular resolution.
DOI: 10.1038/s41592-020-01055-6
Source: https://www.nature.com/articles/s41592-020-01055-6
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